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KMID : 0366120010290010050
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Korean Journal of Applied Microbiology & Bioengineering
2001 Volume.29 No. 1 p.50 ~ p.55
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Characterization of Bacteriocin from Bacillus subtilis cx1
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Kim Soo-In
Chang Ji-Yoon
Kim In Cheol
Chang Hae-Choon
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Abstract
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A new bacteriocin produced by Bacillus subtilis cx 1, was partially purified and characterized. The bacteriocin from B. subtilis cx1 was stable in the range of pH 2.5~9.5. B. subtilis cx1 retained its antimicrobial activity to long-term exposure at -20℃ and -70℃. However, B. subtilis cx1 was inactivated completely within 15 min over 60℃ and lost 50% of its antimicrobial activity within 15 min at 50℃. B. subtilis cx1 was inactivated by protease, trypsin, proteinase K and carboxypeptidase, which indicates its protein nature. Direct detection of the antimicrobial activity on Tricine-SDS-PAGE suggested an apparent molecular mass of about 9,500 dalton.
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KEYWORD
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Bacillus subtilis cx1, bacteriocin
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